핵심기술
특허 / 논문
| Mapping the human CAS2 gene, the homologue of the mouse brown (b) locus, to human chromosome 9P22-pter. |
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| 등록일 :2022-10-31 11:02 조회수 :201 |
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Mapping the human CAS2 gene, the homologue of the mouse brown (b) locus, to human chromosome 9P22-pter. Chintamaneni C, Ramsay M, Colman MS, Fox MF, Pickard RT, Kwon BS
Abstract Melanin biosynthesis is a multistep process with the first step being the conversion of L-tyrosine to L-Dopa catalyzed by the enzyme tyrosinase. The enzymes which catalyze the other steps of melanogenesis are not known. One murine pigmentation gene, the brown (b) locus, when mutated, leads to a brown or hypopigmented coat. The b-locus protein has been shown to display catalase activity. The human b-locus, therefore, is designated as CAS2. We used the mouse b-locus cDNA to isolate the human homologue, which in turn, was used to map the CAS2 locus to a human chromosome. The potential CAS2 protein codes for 527 amino acids containing a putative signal sequence and transmembrane domain. The CAS2 protein has primary and probably secondary structures similar to human tyrosinase. The CAS2 was mapped to human Chromosome 9 by somatic cell hybridization and, more specifically, to 9p22-pter by in situ hybridization. The assignment of CAS2 on the human Chromosome 9 extends this region of known homology on mouse Chromosome 4.
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