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4-1BB is expressed on CD45RAhi CD45ROhi transitional T cell in humans.
4-1BB is expressed on CD45RAhi CD45ROhi transitional T cell in humans. Beth AnnGarni-Wagner, Zang H.Lee, Young-JuneKim, CharlesWilde, Chang-YKang, Byoung S.Kwon Abstract Murine 4-1BB is an ∼30-kDa glycoprotein expressed on activated T cells and plays a role in T-cell-mediated proliferative response. To date the majority of work on 4-1BB has been conducted in the mouse. To assess the role of 4-1BB in humans, mAbs were made against the recombinant human (rh) 4-1BB protein. One such mAb 4B4-1 specifically binds SF-21 insect cells expressing rh4-1BB but not irrelevant control protein as measured by flow cytometry (FCM). 4B4-1 mAb stains PMA- and ionomycin-stimulated CEM (human T lymphoma) cells and PHA-stimulated peripheral blood T cells, but not resting cells. 4B4-1 mAb immunoprecipitates both ∼32 and ∼80 kDa protein from rh4-1BB expressing SF-21 cells and an ∼39- and ∼85-kDa protein from PMA-stimulated CEM cells under reducing conditions by SDS–PAGE. As added proof of its specificity, binding of FITC-labeled 4B4-1 mAb to PHA-stimulated T cells was blocked by rh4-1BB protein. Together these data demonstrate that 4B4-1 is specific for 4-1BB in humans. Unlike in the mouse, 4-1BB is expressed much earlier (within 24 hr) peaking around 2–3 days following PHA stimulation. As in the mouse 4-1BB is induced on both CD4+and CD8+T cell subsets. 4-1BB expression is induced upon PHA stimulation in both the naive (CD45RAhi-CD45ROlo/−) and the memory (CD45RAlo/−- ROhi) T cell populations. Virtually all CD45RAhiROlo/−cells upon culture in PHA give rise to an intermediate CD45RAhiROhi4-1BB+transitional cell and subsequently CD45RAlo/−ROhi4-1BBlo/−and CD45RAlo/−ROhi4-1BBhicells. In contrast, approximately 27% of CD45RAlo/−ROhi4-1BB−cells when cultured in PHA for 24 hr acquire 4-1BB expression and all remain CD45RAlo/−ROhi. 논문 링크(클릭시 이동)

4-1BB is expressed on CD45RAhi CD45ROhi transitional T cell in humans.

등록일 :2022-10-31 16:04 조회수 :234

4-1BB is expressed on CD45RAhi CD45ROhi transitional T cell in humans.

Beth AnnGarni-Wagner, Zang H.Lee, Young-JuneKim, CharlesWilde, Chang-YKang, Byoung S.Kwon

Abstract

Murine 4-1BB is an ∼30-kDa glycoprotein expressed on activated T cells and plays a role in T-cell-mediated proliferative response. To date the majority of work on 4-1BB has been conducted in the mouse. To assess the role of 4-1BB in humans, mAbs were made against the recombinant human (rh) 4-1BB protein. One such mAb 4B4-1 specifically binds SF-21 insect cells expressing rh4-1BB but not irrelevant control protein as measured by flow cytometry (FCM). 4B4-1 mAb stains PMA- and ionomycin-stimulated CEM (human T lymphoma) cells and PHA-stimulated peripheral blood T cells, but not resting cells. 4B4-1 mAb immunoprecipitates both ∼32 and ∼80 kDa protein from rh4-1BB expressing SF-21 cells and an ∼39- and ∼85-kDa protein from PMA-stimulated CEM cells under reducing conditions by SDS–PAGE. As added proof of its specificity, binding of FITC-labeled 4B4-1 mAb to PHA-stimulated T cells was blocked by rh4-1BB protein. Together these data demonstrate that 4B4-1 is specific for 4-1BB in humans. Unlike in the mouse, 4-1BB is expressed much earlier (within 24 hr) peaking around 2–3 days following PHA stimulation. As in the mouse 4-1BB is induced on both CD4+and CD8+T cell subsets. 4-1BB expression is induced upon PHA stimulation in both the naive (CD45RAhi-CD45ROlo/−) and the memory (CD45RAlo/−- ROhi) T cell populations. Virtually all CD45RAhiROlo/−cells upon culture in PHA give rise to an intermediate CD45RAhiROhi4-1BB+transitional cell and subsequently CD45RAlo/−ROhi4-1BBlo/−and CD45RAlo/−ROhi4-1BBhicells. In contrast, approximately 27% of CD45RAlo/−ROhi4-1BB−cells when cultured in PHA for 24 hr acquire 4-1BB expression and all remain CD45RAlo/−ROhi.

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