핵심기술
특허 / 논문
| An Intronic silencer of the mouse perforin gene. |
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| 등록일 :2022-11-01 16:53 조회수 :215 |
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An Intronic silencer of the mouse perforin gene.
Youn BS, Lim CL, Shin MK, James MH and Kwon BS
Abstract We have previously shown that the perforin gene locus is comprised of eight DNase I hypersensitive sites (DHS). Seven (DHS I-DHS VII) of them were CTL-specific whereas one (DHS VIII) in the second intron was expressed in a wide range of cell types. DHS VIII was highly AT-rich (75%) and was comprised of multiple sets of high mobility group (HMG)-I/Y binding site, two potential Special AT-rich Binding protein (SATB-1)-binding sites, and a long stretch of CTAT repeats, indicating that DHS VIII may relate to nuclear matrix-associated region (MAR). When DHS VIII was inserted into the perforin promoter-driven luciferase gene, it silenced the reporter gene transcription in CTLL-R8 cells in an orientation- and distance-independent manner. Moreover, this silencing effect was also observed in other promoters in a variety of non-CTL cell lines, suggesting that DHS VIII exerted a global silencing effect. Deletion analysis and gel-shift assays indicated that the silencing effect was mediated by the CTAT repeats and its binding protein called CTAT repeats-binding protein (CRBP). |