핵심기술
특허 / 논문
| Changes in gene expression in latent HSV-1-infected rabbit trigeminal ganglia following epinephrine iontophoresis. |
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| 등록일 :2022-11-02 12:39 조회수 :214 |
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Changes in gene expression in latent HSV-1-infected rabbit trigeminal ganglia following epinephrine iontophoresis.
Kang SW, Seo SK, Hill JM, Kwon B, Lee HW, Cho HR, Vinay DS, Kwon BS
Abstract Purpose: The aim of the present study was to identify the genes that express in the nervous system when herpes simplex virus type 1 (HSV-1) reactivates from latency by using subtraction cloning following epinephrine iontophoresis. Methods: The corneas of New Zealand White rabbits (2 to 2.5 kg) were topically inoculated with 5 x 10(5) PFU of plaque-purified HSV-1 strain McKrae. Corneal infection was monitored by slit lamp examination (SLE) on days 3-7 after infection. At 30 d post-infection, eyes were assessed for infectious virus by ocular swabs. Disappearance of virus in ocular swabs signified latency for HSV-1. Latently-infected rabbits were subjected to transcorneal iontophoresis. The messenger RNA (mRNA) was isolated from the trigeminal ganglia and used for subtraction cloning and subtraction library synthesis. Results: Our results indicate 18 genes with increased expression and 6 genes with decreased levels. Northern blot analysis revealed that genes for OX-40 ligand, MHC class II HLA-DR-alpha and beta-microglobulin showed predominant increase followed by tyrosine 3'-monooxygenase, proteolipid protein, tyrosine beta, and apolipoprotein D. Genes that underwent reduced expression included cytochrome c oxidase subunit I, ATP synthase isoform, cytochrome b, ATPase 9, and at least one unidentified gene. Conclusions: Our results, particularly the increased detection of OX-40 ligand and MHC class II-alpha and beta-2 microglobulin, support the finding that lymphocytes persist in latently-infected trigeminal ganglia (TGs). These results also suggest a role of the immune system in HSV-1 recurrences. In addition, we have detected genes associated with neuronal disorders, OX-40L, proteolipid protein, and apolipoprotein D.
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